&MULTIPLE_FORCE_EVALS
   FORCE_EVAL_ORDER 2 3 4 5
   MULTIPLE_SUBSYS T
&END
&FORCE_EVAL
  STRESS_TENSOR ANALYTICAL
  METHOD MIXED
  &MIXED
    MIXING_TYPE GENMIX
    &GENERIC
      MIXING_FUNCTION k*X+(1.0-k)*Y+(1.0-k)*Z+k*J
      VARIABLES X Y Z J
      PARAMETERS k
      VALUES 0.5
    &END
    &MAPPING
      &FORCE_EVAL_MIXED
        &FRAGMENT 1
          1 6
        &END
        &FRAGMENT 2
          7 16
        &END
        &FRAGMENT 3
          17 22
        &END
        &FRAGMENT 4
          23 33
        &END
        &FRAGMENT 5
          34 1851
        &END
      &END
      &FORCE_EVAL 1
        &FRAGMENT 1
          1 6
          MAP 1
        &END
        &FRAGMENT 2
          7 16
          MAP 2
        &END
        &FRAGMENT 3
          17 22
          MAP 3
        &END
        &FRAGMENT 4
          23 1840
          MAP 5
        &END
      &END
      &FORCE_EVAL 2
        &FRAGMENT 1
          1 6
          MAP 1
        &END
        &FRAGMENT 2
          7 17
          MAP 4
        &END
        &FRAGMENT 3
          18 23
          MAP 3
        &END
        &FRAGMENT 4
          24 1841
          MAP 5
        &END
      &END
      &FORCE_EVAL 3
        &FRAGMENT 1
          1 11
          MAP 4
        &END
      &END
      &FORCE_EVAL 4
        &FRAGMENT 1
          1 10
          MAP 2
        &END
      &END
    &END
  &END
  &SUBSYS
    &TOPOLOGY
      CONNECTIVITY OFF
      COORDINATE PDB
      COORD_FILE_NAME ../regtest-1/full.pdb
    &END TOPOLOGY
    &CELL
      ABC  30.9751780  30.8599250  30.7278700
    &END CELL
  &END SUBSYS
&END FORCE_EVAL
&FORCE_EVAL
  STRESS_TENSOR ANALYTICAL
  METHOD FIST
  &MM
    &FORCEFIELD
      PARM_FILE_NAME ../regtest-1/ala.pot
      parmtype CHM
      &SPLINE
        EMAX_SPLINE 100000000.0
      &END
    &END FORCEFIELD
    &POISSON
      &EWALD
        EWALD_TYPE spme
        ALPHA .5
        GMAX 36
        O_SPLINE 6
      &END EWALD
    &END POISSON
  &END MM
  &SUBSYS
    &TOPOLOGY
      CONNECTIVITY PSF
      CONN_FILE_NAME ../regtest-1/ala.psf
      COORDINATE PDB
      COORD_FILE_NAME ../regtest-1/ala.pdb
    &END TOPOLOGY
    &CELL
      ABC 30.9751780  30.8599250  30.7278700
    &END CELL
  &END SUBSYS
&END FORCE_EVAL
&FORCE_EVAL
  STRESS_TENSOR ANALYTICAL
  METHOD FIST
  &MM
    &FORCEFIELD
      PARM_FILE_NAME ../regtest-1/ser.pot
      parmtype CHM
      &SPLINE
        EMAX_SPLINE 100000000.0
      &END
    &END FORCEFIELD
    &POISSON
      &EWALD
        EWALD_TYPE spme
        ALPHA .5
        GMAX 36
        O_SPLINE 6
      &END EWALD
    &END POISSON
  &END MM
  &SUBSYS
    &TOPOLOGY
      CONNECTIVITY PSF
      CONN_FILE_NAME ../regtest-1/ser.psf
      COORDINATE PDB
      COORD_FILE_NAME ../regtest-1/ser.pdb
    &END TOPOLOGY
    &CELL
      ABC  30.9751780  30.8599250  30.7278700
    &END CELL
  &END SUBSYS
&END FORCE_EVAL
&FORCE_EVAL
  STRESS_TENSOR ANALYTICAL
  METHOD FIST
  &MM
    &FORCEFIELD
      DO_NONBONDED F
      PARM_FILE_NAME ser_s.pot
      parmtype CHM
      &SPLINE
        EMAX_SPLINE 100000000.0
      &END
    &END FORCEFIELD
    &POISSON
      &EWALD
        EWALD_TYPE none
      &END EWALD
    &END POISSON
  &END MM
  &SUBSYS
    &TOPOLOGY
      CONNECTIVITY PSF
      CONN_FILE_NAME ser_s.psf
      COORDINATE PDB
      COORD_FILE_NAME ser_s.pdb
    &END TOPOLOGY
    &CELL
      ABC  30.9751780  30.8599250  30.7278700
    &END CELL
  &END SUBSYS
&END FORCE_EVAL
&FORCE_EVAL
  STRESS_TENSOR ANALYTICAL
  METHOD FIST
  &MM
    &FORCEFIELD
      DO_NONBONDED F
      PARM_FILE_NAME ala_s.pot
      parmtype CHM
      &SPLINE
        EMAX_SPLINE 100000000.0
      &END
    &END FORCEFIELD
    &POISSON
      &EWALD
        EWALD_TYPE none
      &END EWALD
    &END POISSON
  &END MM
  &SUBSYS
    &TOPOLOGY
      CONNECTIVITY PSF
      CONN_FILE_NAME ala_s.psf
      COORDINATE PDB
      COORD_FILE_NAME ala_s.pdb
    &END TOPOLOGY
    &CELL
      ABC  30.9751780  30.8599250  30.7278700
    &END CELL
  &END SUBSYS
&END FORCE_EVAL
&GLOBAL
  PROJECT DDG_SOLVATION
  RUN_TYPE MD
  PRINT_LEVEL LOW
&END GLOBAL
&MOTION
  &MD
    ENSEMBLE NPT_I
    STEPS 10
    TIMESTEP 0.5
    TEMPERATURE 200.0
    &THERMOSTAT
      &NOSE
        LENGTH 3
        YOSHIDA 3
        TIMECON 300
        MTS 2
      &END NOSE
    &END
    &BAROSTAT
    &END
    &PRINT
     &ENERGY
      &EACH
       MD 1
      &END
     &END
    &END
  &END MD
  &PRINT
   &TRAJECTORY
    &EACH
      MD 100
    &END
   &END
   &VELOCITIES OFF
   &END
   &FORCES OFF
   &END
   &RESTART_HISTORY OFF
   &END
   &RESTART
    &EACH
     MD 10
    &END
   &END
  &END
  &FREE_ENERGY
    METHOD AC
    &ALCHEMICAL_CHANGE
      PARAMETER k
      WEIGHTING_FUNCTION (1.0-k)*Z+k*J
    &END
  &END
&END MOTION
